Derek

__**05 October 2011**__ -Reviewed basic molecular terms and functions. -Began RNA isolation procedures on C. gigas gill tissue to protocol, stopped procedure after homogenization step. -Ran protein isolation procedure on C. gigas digestive gland tissue using CelLytic MT and Bradford Assay. -Learned how to operate the spectrophotometer.
 * Summary:**

-Will finish at a later date.
 * Methods and Materials:**

1. RNA Isolation procedure used 0.02g C. gigas gill tissue. -Added a total of 1000ul of Tri-Reagent 2. Protein Extraction procedure used 0.053g of digestive gland tissue. -For protein extraction: Concentration - 530.27ug/ml Calculation - y=mx+b m=1013.9 x=0.2615 * 2 (to account for the dilution) b=0
 * Recordings and Measurments:**
 * Sample || Reading ||
 * First absorbance || 0.261 ||
 * Second absorbance || 0.262 ||
 * Average || 0.2615 ||
 * Results:**

y=1013.9(2*0.2615) y=530.27ug/ml

-RNA isolation went as expected and there is not much detail to give until the procedure is completed. -Protein extraction results showed a high concentration of protein within the digestive gland. Something to compare would be this concentration to the other tissue samples as I expect 530.27ug/ml to be a high concentration level.
 * Conclusion:**

-Complete RNA isolation procedures. -Compare concentration levels from other groups in lab. -Begin putting group project ideas together.
 * Next Step:**

Purpose: -To understand and perform basic fundamental techniques ( RNA isolation and Protein Extraction) used in molecular biology.
 * Reflection:**

-The RNA isolation procedures in lab are used to measure the amount of mRNA being produced by the organism or the change in expression. -The protein extraction procedure measures the concentration of protein being secreted by the organism.