Week1

toc **Sept 29 ||  || Introduction Week 1 Sept 29
 * ** Introduction Week 1

Introduction Discussion of discussions: format

Science 2.0

**Molecular 101**
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What is PCR?
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Paper
[|ncb0908-1015.pdf]

Schedule of Topics
Week 1 - Intro Week 2 - Gene Discovery (Amanda & Kristy) Week 3 - Bioinformatics (Lisa & Mac) Week 4 - Epigenetics (Jennifer & Amanda) Week 5 - Next Generation Sequencing Technology (Bethany & Lisa) Week 6 - Molecular Diagnostics (Jess & Carl) Community Ecology (Jess & Jennifer) Week 7 - QRT-PCR (Angela & Carl) Week 8 - Microarrays (Mac & Bethany) Week 9 - Proteomics (John & Kristy) Week-10 - Metabolomics (John & Angela)

Molecular Discovery [small scale] Differential Display, Suppression Subtraction Hybridization, Degenerative PCR Applications: various, environmental effects, physiology, explanation of phenotype Molecular Discovery [large scale] Sequencing: Expressed Sequence Tags (ESTs), enriched microsat library, genomes Applications: various, environmental, stock assessment, parentage, marker ID, phylogeny Bioinformatics gene discovery, BLAST, sequence assembly Applications: in silico gene discovery / genetic markers, routine analysis of sequences, Large Scale Expression Analysis SAGE, 454, illumina, array Applications: developmental changes, environmental effects, marker discovery, 16s rRNA diversity QPCR SYBR Green, Taqman Applications: microbial abundance/diversity, disease diagnostic, plankton tow/ larval ID+ abundance, SNPS)  QRTPCR  SYBR Green, Taqman  Applications: developmental larval tracking, physiology, various, explanation of phenotype   Population Genetic Marker Analysis  SSR, SNPs  Applications: stock structure, parentage, reproductive success, distribution patterns, genetic diversity   Protein Analysis  [proteome] Commassi gel, 2-D electrophoresis, sequencing  Applications: kin recognition, physiology, environmental effects, phenotype explanation, microbial signatures   Protein Analysis  [targeted] Western analysis, yeast two hybrid protein-protein interaction  Applications: physiology, disease mechanisms, cellular processes, stress indicators, Gene / Protein Expression [tissue/cell localization] Histology, immunocytochemistry, in-situ Applications: physiology, disease diagnostics, developmental differences
 * Technique Sampling**

