Week5

=**Week 5**= October 27

=**Next Generation Sequencing**=

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[|How High-Speed Gene Sequencing Works] [via Wired Science] //NNGS 1225246364//
= = rss url="http://feeds.delicious.com/v2/rss/sr320/ngs?count=15" link="true" number="10" [|full list from delicious]

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> Different sequencing technologies, at a glance. NATURE METHODS | VOL.5 NO.1 | JANUARY 2008 | 15 > 6/1/2008 > > PLoS ONE. 2008; 3 (8) : e3042.
 * Papers to Discuss**:
 * **[|Primer: Sequencing—the next generation]**
 * **[|Pyrosequence analysis of expressed sequence tags for Manduca sexta hemolymph proteins involved in immune responses.]** Zou Z, Najar F, Wang Y, Roe B, Jiang H. Insect Biochemistry and Molecular Biology Volume38, Ppg. 677-82
 * **[|Detection of Large Numbers of Novel Sequences in the Metatranscriptomes of Complex Marine Microbial Communities]**Jack A. Gilbert,1* Dawn Field,2 Ying Huang,3 Rob Edwards,4,5 Weizhong Li,3 Paul Gilna,3 and Ian Joint1

Special Guest Presentations | Dr. Jim Seeb and Dr. Lisa Seeb
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**The Basics:** [|DNA sequencing] Traditional (Sanger)
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[|Early DNA sequencing (Sanger)]

NGS
[|Solexa Technology]

[|Two movies from Roche] (one hardware, the other on chemistry)



> Stephan C Schuster | 18 | VOL.5 NO.1 | JANUARY 2008 | NATURE METHODS > blog.wired.com/wiredscience/2008/07/british-ins... > 6/1/2008 > 6/28/2008 > [| View Publication] > 2/5/2008 > [| View Publication] > 3/11/2008 > [| View Publication] > 6/25/2008
 * Additional Readings on the topic:**
 * **[|Next-generation sequencing transforms today’s biology]**
 * **[|DNA Technology Posts Dramatic Speed Increases]** | Wired Science from Wired.com
 * **[|Pyrosequence analysis of expressed sequence tags for Manduca sexta hemolymph proteins involved in immune responses.]** Zou Z, Najar F, Wang Y, Roe B, Jiang H. Insect Biochemistry and Molecular Biology Volume38, Ppg. 677-82
 * **[|Construction of a robust microarray from a non-model species largemouth bass, Micropterus salmoides (Lacèpede), using pyrosequencing technology]** N. Garcia-Reyero, R. J. Griffitt, L. Liu, K. J. Kroll, W. G. Farmerie, D. S. Barber, N. D. Denslow Journal of Fish Biology Volume72, Ppg. 2354 - 2376
 * **[|Rapid transcriptome characterization for a non-model organism using 454 pyrosequencing]** J. C. Vera, C.W. Wheat, H.W. Fescemyer, M.J. Frilander, D.L. Crawford, I. Hanski, J.H. Marden Molecular Ecology Volume17, Ppg. 1636-1647
 * **[|Microbial community gene expression in ocean surface waters]** Jorge Frias-Lopez, Yanmei Shi, Gene W. Tyson, Maureen L. Coleman, Stephan C. Schuster, Sallie W. Chisholm, and Edward F. DeLong PNAS Volume105, Ppg. 3805–3810
 * **[|Simultaneous assessment of soil microbial community structure and function through analysis of the meta-transcriptome]** Urich T, Lanzén A, Qi J, Huson DH, Schleper C, Schuster SC. PloS One Volume3, Ppg.
 * [|doi:10.1016/j.ygeno.2008.07.001] Genomics Volume 92, Issue 5, November 2008, Pages 255-264**[|> Review Applications of next-generation sequencing technologies in functional genomics]**

**Cool Links**
[|biocompare]