NGS+Protocols


 * More info including data and discussion @......[[image:http://img.skitch.com/20091124-qjmd6dkrjkku2mbhgt5jq6r6ce.jpg width="284" height="220" align="left" link="@http://genefish.fish.washington.edu/wetgenes/home.html"]]. || **TOC**flat ||

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= = = = =Commercial Protocols= [|Ribominus Kit] (Invitrogen) [|Ribominus Sample Concentration Kit] (Invitrogen) [|SOLiD WTK] (AB) [|SOLiD WTK Quick Reference Card] (AB) [|SOLiD 3+ Templated Bead Preparation Guide] (AB) [|SOLiD 3+ Instrument Operation Guide October 2009] (AB)

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=BioAnalyzer= Nano vs Pico Using less sample is the main advantage; however, the top end of the Pico range is pretty low so it is often easier for people with plenty of RNA to submit undiluted samples for the Nano assay. Additionally, while the Bioanalyzer is considered only semi-quantitative, the Nano assay has better quantitative accuracy than the Pico assay (20%CV within chip for Nano versus 30%CV within chip for Pico). The acceptable concentration ranges of the Nano versus Pico are:

Nano: 25ng/ul-500ng/ul (Total) or 50ng/ul-500ng/ul (mRNA) Pico: 50pg/ul-5ng/ul (Total) or 250pg/ul-5ng/ul (mRNA)

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